Composite
Part:BBa_K2957039
Designed by: Ethan Levy Group: iGEM19_MIT (2019-10-20)
hEF1a, mKate
Contents: hEF1a, mKate, SynthpA, with Amp resistance
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and mKate reporter gene.
What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu
How to use it?: This part was used for transfection into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (a href=https://2019.igem.org/Team:MIT/Parts)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 968
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 968
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 968
Illegal AgeI site found at 81
Illegal AgeI site found at 974 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |